SaProtHub/Dataset-Meta-scale-protein-stability

Description

This dataset contains ΔΔG value of various protein mutations (including some human designed proteins). The ΔΔG value indicates the stability of protein.

Protein Format: AA sequence

Splits

traing: 486352

valid: 60995

test: 60492

Related paper

Tsuboyama, K., Dauparas, J., Chen, J. et al. Mega-scale experimental analysis of protein folding stability in biology and design. Nature 620, 434–444 (2023). https://doi.org/10.1038/s41586-023-06328-6.

Label

Label means ΔΔG of each mutant amino acid sequence based on a deep mutation scanning experiment.

Understanding ΔG (delta G)

ΔG is the Gibbs free energy change of a process, dictating whether a process is thermodynamically favorable:

Negative ΔG: Indicates the process is energetically favorable. For protein unfolding, it implies the protein is more stable in its unfolded form.

Positive ΔG: Indicates the process is not energetically favorable. In protein unfolding, it means the protein requires energy to maintain its unfolded state, i.e. it is stable in folded form.

The delta delta G (ΔΔG) represents the deltaG of the mutation compared to the base protein:

Positive ΔΔG: Suggests the mutation enhances protein stability.

Negative ΔΔG: Suggests the mutation decreases protein stability.