Jumbol/Acr_Prophage_External_Validation_Dataset
Prophage ACR Screening Pipelines This directory contains two analysis pipelines for screening Anti-CRISPR (Acr) proteins from prophage genomes. Directory Structure Folder Description double_diff/ Double-divergence screening pipeline: identifies novel Acr candidates that are significantly different from known Acr proteins at both sequence and structure levels double_similar/ High-confidence Acr identification pipeline: identifies high-confidence Acr… See the full description on the dataset page: https://huggingface.co/datasets/Jumbol/Acr_Prophage_External_Validation_Dataset.
Prophage ACR Screening Pipelines
This directory contains two analysis pipelines for screening Anti-CRISPR (Acr) proteins from prophage genomes.
Directory Structure
Quick Navigation
- [double_diff](double_diff/README.md) — For discovery of Acrs with novel mechanisms. Excludes proteins with sequence homology (qcovs≥30, pident≥30) or structural similarity to known Acrs, retaining "double-divergent" candidates.
- [double_similar](double_similar/README.md) — For expansion of known Acr families. Applies stringent filters for sequence (qcovs≥50, pident>50) and structural (TM-score>0.7, prob=1.0) similarity to obtain high-confidence candidates.
Detailed Documentation
Each folder contains a comprehensive README document describing:
- Screening strategies and workflows
- Input and output data formats
- Software tools and parameter settings
- Screening thresholds and criteria
Click the links above to view detailed documentation for each pipeline.