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Evenli2026/uce-spleen-granulocyte-NeuEosBaso

Granulocyte lineage split: Neutrophil vs Eosinophil vs Basophil Rad54b KO vs WT mouse spleen scRNA-seq. Who drives the KO granulocyte expansion? Verdict Neutrophil-dominated (primary share of positive Δ ≈ 96.3%; after construct-validity raw_ratio_gate ≈ 99.9%). Eosinophils rare; basophils essentially absent. Skills: scientific-critical-thinking, statistical-analysis, scanpy. How to read FigS_Granulocyte_NeuEosBaso_score_UMAP Three panels share the… See the full description on the dataset page: https://huggingface.co/datasets/Evenli2026/uce-spleen-granulocyte-NeuEosBaso.

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Granulocyte lineage split: Neutrophil vs Eosinophil vs Basophil

Rad54b KO vs WT mouse spleen scRNA-seq. Who drives the KO granulocyte expansion?

Verdict

Neutrophil-dominated (primary share of positive Δ ≈ 96.3%; after construct-validity raw_ratio_gate ≈ 99.9%). Eosinophils rare; basophils essentially absent.

Skills: scientific-critical-thinking, statistical-analysis, scanpy.

How to read FigS_Granulocyte_NeuEosBaso_score_UMAP

Three panels share the same UMAP coordinates (UMAP1 = x, UMAP2 = y). Axes are only map positions — not biology units.

Color = lineage signature z-score within granulocytes (title “(z)”):

  • —yellow/bright = relatively high for that panel’s markers
  • —purple/dark = relatively low
  • —colorbar scales differ across panels — do not compare absolute color across Neu vs Eos vs Baso

Interpretation: Neutrophil panel shows broad high signal; Eosinophil mostly low with rare bright cells; Basophil nearly flat (no real program).

Key caveats

  • —n=3 vs 3 → MW floor p=0.10; report Cliff’s δ / log2FC, not FDR discovery
  • —Z-only “Eosinophil” calls still have higher Neutrophil raw scores → false Eos; raw-ratio gate removes them

Contents

  • —figures/ — UMAP, signature scores, boxplots, contribution, audit plots
  • —tables/ — stats, sensitivity, LOO, construct validity, JSON summary
  • —audit/ — full rigor report
  • —RESULTS_section15.md — manuscript-facing summary
  • —NeuEosBaso_rigor_bundle.zip — full pack

Updated: 2026-08-08

Related

  • —Mechanistic 5-module plan: https://huggingface.co/datasets/Evenli2026/uce-spleen-mechanistic-logic