SkyWhal3/stxbp1_human_to_mouse_base-editing_context_verifier

STXBP1 Human↔Mouse Base-Editing Context Verifier

This Space compares human vs mouse STXBP1 CDS context around user-specified variants and scans for SpCas9 NGG PAM candidate guides near each variant.

What it does

• —Fetches RefSeq mRNA CDS from NCBI (cached after first run)
• —Calculates variant position in the CDS from c. notation
• —Extracts a context window around the variant and reports mismatches
• —Scans for nearby NGG PAMs and reports protospacer/PAM conservation

What it doesn't do

• —No off-target prediction, efficiency scoring, chromatin modeling, or delivery constraints
• —Not medical advice / not a clinical tool

Input format

Paste lines like:

• —R43W c.127C>T p.Arg43Trp
• —c.586A>T

Or provide a JSON list:

[
  {"label":"R43W","cdna":"c.127C>T","aa":"p.Arg43Trp"}
]

Credits

Built from Adam's verifier script (v3) adapted to a Gradio Space.

Dependency note

This Space pins huggingface_hub<1.0.0 to keep compatibility with Gradio OAuth imports (Gradio currently imports HfFolder from huggingface_hub).